Part:BBa_K4131008:Design

pfa B gene (Shewanella pneumatophori), codon optimized for E.Coli expression

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1305
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 297
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1515
  

Design Notes

The sequence is codon optimized for E.Coli expression, and the restriction enzyme sites of XbaI, SpeI, EcoRI, PstI, and NotI are eliminated.

Source

The sequence is extracted from the region 22177-24518 in Shewanella pneumatophori strain SCRC-2738 genome (U73935.1)